本教材是針對新形勢下的教學改革，為適應中國與國際接軌，高等院校普及雙語教學的需要而編寫的。本教材根據(jù)醫(yī)學免疫學與微生物學的教學要求，遵循實用性、科學性的原則，從實驗目的、材料、方法、結果分析及思考題等方面入手，較為系統(tǒng)和全面地介紹醫(yī)學免疫學及醫(yī)學微生物學的基本實驗技術。全教材分為兩篇：第一篇為醫(yī)學免疫學實驗；第二篇為醫(yī)學微生物學實驗。本教材培養(yǎng)學生獨立操作、觀察和思考，分析問題和解決實際問題的能力。
江振友,男,1963年9月生，暨南大學醫(yī)學院微生物學與免疫學系系主任、教授、博士生導師。中國微生物學會醫(yī)學微生物學與免疫學專業(yè)委員會委員；廣東省免疫學會第四屆理事會理事；廣東省防癆協(xié)會第八屆理事會常務理事；廣東省微生物學會第九屆理事會理事。主要從事分子病毒學、感染免疫、病原生物感染診斷研究。近年發(fā)表論文60余篇，其中SCI收錄20多篇。主編或參編著作5部。獲得各類科研研究獎勵4項，教學研究獎勵6項。主持承擔國家、省部級等各類科研課題6項,研究經(jīng)費300多萬元。授權發(fā)明專利3項。
前言
Preface
Part1MedicalImmunologyExperiment
Instructions
i．PurposesandRequirementsoftheExperiment
ii．RulesandRegulationsoftheLaboratory
I．AgglutinationReaction
i．DirectAgglutinationReaction
ii．IndirectAgglutinationReaction
II．PrecipitationReaction·
i．SingleAgarImmunodiffusionTest
ii．DoubleAgarhnmunodiffusionTest
iii．CounterImmunoelectrophoresisTest
iv．ImmunoelectrophoresisTest
v．RocketElectrophoresisTest
III．PhagocytosisTest
i．ThePhagoeytosisofMacrophage--BigPhagocytosisPhenomenon
ii．NeutrophilPhagocytosis--SmallPhagocytosisPhenomenon(InVitro)
IV．ThePreparationofMouseSplenocyte
V．HemolyticPlaqueFormationTest
VI．E-RosetteFormingCellTest
VII.TLymphocyteTransformationTest
VIII.ImmunolabellingTechnique
i．DetectionofBLymphocyteAntigenRecognitionReceptors(Smlg)(DirectImmunofluorescenceMethod
ii．Enzyme-LinkedImmunosorbentAssay(ELISA)
IX．AnaphylacticShockinGuineaPig
Part2MedicalMicrobiologyExperiment
X．MorphologyandStructureoftheBacteria
XI．ObservationofUnstainedBacteriaSpecimen
XII．ObservationofStainedBacteriaSpecimen
i．GramsStaining
ii．Acid-FastStaining
XIII．ArtificiaICultureofBacteria
i．InoculationMethodsforArtificialCultureofBacteria
ii．BiochemicalReactionofBacteria
XIV.TheDistributionOfBacteria
i．BacterialExaminationintheNaturalEnvironment
ii．NormalHumanBacterialExamination
XV．TheInfluenceofExternalFactorsonBacteria
i．TheInfluenceofPhysicalFactorsonBacteria
ii．TheInfluenceofChemicalFactorsonBacteria
iii．Test0nBacterialSensitivitytoAntibiotics(AgarDiffusionMethod)
XVI．TheSpecificBacteriolyticTestofBacteriophage
XVII．PathogenicityTestofPathogenicBacteria
i．DiffusionEffectofHyaluronidase
ii．PlasmaCoagulaseTest
iii．DetectionofEndotoxinandLimulusTest
XVIII．LysozymeTest
XIX．PathogenicCocci
i．TheMorphologyandStainingCharacteristicsofStaphylococcus，Streptococcus，StreDtococcMsPneumoniae，NeisseriaMeningitidisandGonococcus
ii．TheCultureCharacteristicsofStaphylococcus，StreptococcusandStreptococcusPneumoniae
iii．Anti-StreptolysinO(ASO)Detection
XX．Enterobacteriaceae
i．TheMorphologyandStainingCharacteristicsofEnterobacteriaceae
ii．CultureCharacteristicsofEnterobacteriaceae
iii．BiochemicalReactionsofEnterobacteriaceae
XXI．TheSeparationandIdentificationofPathogenicEnterobacteriaceaeinFecesSpecimens
XXII．TubeAgglutination
XXIII．AnaerobicClostridium
i．AnaerobicCuhure
ii．MorphologyandStainingCharacteristicsofClostridiumTetani，ClostridiumPerfringensandClostridiumBotulinum
XXIV．Mycobacterium
i．TheCuitureCharacteristicsofMycobacteriumTuberculosis
ii．DirectSmearExaminationofSputumSamplesofPulmonaryTuberculosisPatients
XXV．TheMorphologyofSpirochetes(Demo)
XXVI．BasicMorphologyandColonyCharacteristicsofFungi
XXVII．MorphologicalCharacteristicsofViruses
i．ObservationofViralCPE
ii．ObservationofViralInclusionBody
XXVIII．CulturaIMethodsofViruses
i．ChickEmbryoCultureMethod
ii．CellCultureMethod
XXIX．HemagglutinationandHemagglutinationInhibitionTestofInfluenzaVirus
i．HemagglutinationTest
ii．HemagglutinationInhibitionTest
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