Study on the Novelnear-infrared Fluorescent Probes Based on
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- 作者：孫春龍 著
- 出版時間：2019/12/1
- ISBN：9787502481278
- 出 版 社：冶金工業(yè)出版社
- 中圖法分類：TQ464
- 頁碼：
- 紙張：膠版紙
- 版次：
- 開本：16開
近紅外熒光成像技術作為一種非侵入性診斷技術，具有無輻射、無損、實時、在位檢測生物信息等優(yōu)點。菁染料作為近紅外熒光染料中的一類，常被用作熒光檢測標記物或制成熒光成像探針。吲哚七甲川菁染料是菁染料中應用多的一類，由吲哚雜環(huán)、七甲川鏈和N-取代基側(cè)鏈組成，由于其具有避開機體組織自發(fā)熒光干擾和自吸收的能力，當前常被用作熒光成像探針，并成為研究的熱點。而如何提高吲哚七甲川菁染料的光譜性能，光穩(wěn)定性以及將其應用到生物活性檢測當中則成為當前的研究重點�！缎滦瓦胚崞呒状ㄝ碱惤t外熒光探針的設計合成及生物活性研究（英文版）》以吲哚七甲川菁染料為母核，設計、合成了一系列新的熒光探針，其分別具有較好的光穩(wěn)定性、熒光成像性和生物檢測活性等性能，期望本研究的一系列近紅外熒光探針能夠在熒光成像領域中得到廣泛應用。
1 Advances in the Biological Application of Indoles Heptamethine Cyanine Dyes Near-infrared Fluorescent Probes
1．1 Introduction
1．2 pH Application of Sensitive Near-infrared Fluorescent Probe
1．3 Application of Metal lon Selective Near-infrared Fluorescence Probe
1．4 Application of Small Molecule Sensitive Near-infrared Fluorescence Probe
1．5 Application of Labeled Tumor Cell Type Near-infrared Fluorescence Probe
1．6 Application of Near-intfrared Fluorescent Probe in Other Fields
1．7 Summary and Outlook
References
2 The Synthesis of a Near-infrared Fluorescent Probe IR-789 and Its Application in Imaging of MCF-7 Cells
2．1 Abstract
2．2 Introduction
2．3 Materials and Methods
2．3．1 Apparatus and Materials
2．3．2 Synthesis and Characterization of IR
2．3．3 Absorption Analysis
2．3．4 Fluorescence Analysis
2．3．5 MTT Assay
2．3．6 Confocal Imaging
2．4 Results and Discussion
2．4．1 Absorption and Fluorescence Spectra of IR
2．4．2 Test for Probe Selectivity
2．4．3 MTT Assay
2．4．4 Determination of Quantum Yield
2．4．5 Fluorescent Confocal Microscopy Image
2．5 Conclusion
References
3 A Novel Near-infrared Fluorescent Probe IR-736 was Synthesized and its Application in Imaging of Living Cells
3．1 Abstract
3．2 Introduction
3．3 Materials and Methods
3．3．1 Apparatus and Materials
3．3．2 Synthesis and Characterization of IR
3．3．3 Absorption Analysis
3．3．4 Fluorescence Analysis
3．3．5 MTT Assay
3．3．6 Confocal Imaging
3．4 Results and Discussion
3．4．1 The Absorption and Fluorescence Spectra of IR
3．4．2 MTT Assay
3．4．3 Determination of Quantum Yield
3．4．4 Fluorescent Confocal Microscopy Image
3．5 Conclusion
References
4 Synthesis of a Novel Near-infrared Fluorescent Probe IR-780 and Its Application in Imaging of Living Cells
4．1 Abstract
4．2 Introduction
4．3 Materials and Methods
4．3．1 Apparatus and Materials
4．3．2 Synthesis and Characterization of IR
4．3．3 Absorption Analysis
4．3．4 Fluorescence Analysis
4．3．5 MTT Assay
4．3．6 Confocal Imaging
4．4 Results and Discussion
4．4．1 Absorption and Fluorescence Spectra of IR
4．4．2 MTT Assay
4．4．3 Fluorescent Confocal Microscopy Image
4．5 Summary
References
5 Synthesis of a Novel IR-822-Met Near-infrared Probe for in Vivo Tumor Diagnosis
5．1 Abstract
5．2 Introduction
5．3 Materials and Methods
5．3．1 Apparatus and Materials
5．3．2 Synthesis and Characterization of IR-822-Met
5．3．3 Absorption Analysis
5．3．4 Fluorescence Analysis
5．3．5 MTT Assay
5．3．6 In Vitro Targeting Capability of IR-822-Met
5．3．7 Tumor Animal Models
5．3．8 In Vivo Targeting Capability of IR-822-Met
5．4 Results
5．4．1 The Absorption and Fluorescence Spectra of IR-822-Met
5．4．2 MTT Assay
5．4．3 Determination of Quantum Yield
5．4．4 In Vitro Targeting Capability of IR-822-Met
5．4．5 In Vivo Targeting Capability of IR-822-Met
5．4．6 Pathological Study of IR-822-Met
5．5 Conclusions
References
6 Synthesis of a Near-infrared Fluorescent Probe IR-822-DG for Tumor Diagnosis
6．1 Abstract
6．2 Introduction
6．3 Materials and Methods
6．3．1 Apparatus and Materials
6．3．2 Synthesis and Characterization of IR-822-DG
6．3．3 Absorption Analysis
6．3．4 Fluorescence Analysis
6．3．5 MTT Assay
6．3．6 Targeting Ability of IR-822-DG in Vitro Tumor Cells
6．3．7 Tumor Animal Models
6．3．8 Targeting Ability of IR-822-DG in Tumor-Bearing Mice
6．4 Results
6．4．1 Optical Properties of IR-822-DG
6．4．2 MTT Assay
6．4．3 Determination of Quantum Yield
6．4．4 Targeting Ability of IR-822-DG in Vitro Tumor Cells
6．4．5 Targeting Ability of IR-822-DG in Tumor-Bearing Mice
6．4．6 The Efect of the Probe on Tumor Growth and Animal Survival
6．5 Discussion
References
7 A Novel Near-infrared Probe for Imaging of O2 Fluctuations and HtEnhancement in HepG2 Cells
7．1 Abstract
7．2 Introduction
7．3 Experimental
7．3．1 Apparatus and Materials
7．3．2 Design and Sythesis of the Probe IR-747-SAPH
7．3．3 3-（4，5-Dimethythiazo1-2-y1）-2，5-diphenyl Tetrazolium Bromide（MTT） Assay
7．3．4 Cell Culture
7．3．5 Confocal Imaging
7．4 Results and Discussion
7．4．1 Photophysical Properties of IR-747-SAPH
7．4．2 Determination of Ouantum Yield
7．4．3 Selectivity of IR-747-SAPH
7．4．4 MTT Assay
7．4．5 Fluorescence Response Time and Reversibility of IR-747-SAPH
7．4．6 Confocal Fluorescence Images of Living Cells
7．5 Discussion
References
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