第l部分 生物化學實驗基本原理與方法
Part l Basic Principles and Methodologies of BiochemicalExperiments
I生物大分子的分離與純化的一般步驟與方法
I General Procedures and Methods for Isolation andPurification of
Biomacromolecules
11分光光度法
II Spectrophotometry
III層析
III Chromatography
IV電泳
ⅣElectr叩hOresis
V離心
V Centrifugation
V1分子生物學技術基礎——聚合酶鏈式反應(PcR)

前言

第l部分  生物化學實驗基本原理與方法

Part l Basic Principles and Methodologies of BiochemicalExperiments

  I生物大分子的分離與純化的一般步驟與方法

  I General Procedures and Methods for Isolation andPurification of

  Biomacromolecules

  11分光光度法

  II Spectrophotometry

  III層析

  III Chromatography

  IV電泳

  ⅣElectr叩hOresis

  V離心

  V Centrifugation

  V1分子生物學技術基礎——聚合酶鏈式反應(PcR)

  VI Brief Introduction to Basic Technique of MolecularBiologyPolymerase

  Chain Reaction(PCR、

第2部分  基礎性實驗

Part 2 Basic Experiments

  實驗1蛋白質和氨基酸的呈色反應

  Experiment l  Color Reactions of Proteins and AminoAcids

  實驗2蛋白質的沉淀與變性

  Experiment 2 Precipitation and DenaturationofProteins

  實驗3微量凱氏定氮法測蛋白質總氮含量

  Experiment 3 Quantification of Crude Proteins—Kieldahl’SNitrogen

  Determination

  實驗4雙縮脲法測定蛋白質含量

  Experiment 4 Quantification ofProteins—Biulet Method

  實驗5 Folin一酚試劑法測定蛋白質含量

  Experiment 5 Quantification ofProteins———Folin—LowryMethod

  實驗6紫外吸收法測定蛋白質含量

  Experiment 6 Quantification ofProteins by UltravioletAbsorption

  實驗7染料結合法測定蛋白質含量

  Experiment 7 Quantification ofProteins by Dye—bindingMethod

  實驗8幾種重要氨基酸(賴氨酸、色氨酸和脯氨酸)的含量測定

  Experiment 8 Determination of the Contents of SomeImportant Amino Acids

  (LysTrp and Pro)

  實驗9血清蛋白的醋酸纖維薄膜電泳

  Experiment 9 Cellulose Acetate Membrane Electrophoresisof Serum Proteins

  實驗10酪蛋白的制備

  Experiment 10 Preparation ofCasein

  實驗11  SDS聚丙烯酰胺凝膠電泳(PAGE)~IJ定蛋白質分子質量

  Experiment 11 Molecular Weight Determination ofProteinsby SDSPAGE

  實驗12聚丙烯酰胺凝膠等電聚焦測定蛋白質等電點

  Experiment 12 pI Determination of Proteins byPolyacrylamide Gel

  Isoelectrofocusing

  實驗13酶的特性

  Experiment 13 Properties ofEnzymes

  實驗14多酚氧化酶米氏常數(shù)(‰)的測定

  Experiment 14 Determination of Michaelis Constant(Kin)ofPolyphenol

  Oxidase

  實驗1 5核酸酶的活力測定

  Experiment 15 Activity Assay ofNucleases

  實驗16谷丙轉氨酶活力測定

  Experiment 16 Determination ofActivity ofGlutamate—Pyruvate Transaminase

  實驗l 7植物抗氧化酶類(超氧物歧化酶、過氧化物酶和過氧化氫酶)活

  性測定

  Experiment 17 Activity Assay of Common AntioxidantEnzymes(SOD，POD

  and CAT)In Plant

  實驗1 8乳酸脫氫酶(LDH)活性測定

  Experiment 18 Activity Assay ofLactateDehydrogenase(LDH)

  實驗19維生素C的定量測定——2，6二氯酚靛酚法

  Experiment 19 Quantification ofVitamin C by2，6Dichlorophenolindo

  Phenol Method

  實驗20花菜中核酸的分離和鑒定

  Experiment 20 Extraction and Identification ofNucleicAcids From

  Cauliflower

  實驗21酵母核糖核酸的分離及組分鑒定

  Experiment 21 Extraction ofYeast RNA and IdentificationOf Its Components

  實驗22定磷法測定核酸含量

  Experiment 22 Quantification of Nucleic Acids by Methodof Phosphate

  Determination

  實驗23定糖法測定RNA含量

  Experiment 23  Quantification of RNA by RiboseDetermination Method

  實驗24二苯胺法測定DNA含量

  Experiment 24 Quantification of DNA by DiphenylamineMethod

  實驗25紫外吸收法測定核酸含量

  Experiment 25 Quantification ofNucleic Acids byUltraviolet Absorption

  Method

  實驗26醋酸纖維薄膜電泳分離核糖核苷酸

  Experiment 26 Separation of Ribonucleotides by CelluloseAcetate Membrane

  Electrophoresis

  實驗27  還原糖和總糖的含量測定_DNs法

  Experiment 27 Determination of Reducing Sugar and TotalSugar by DNS

  Method

  實驗28血糖的定量測定——葡萄糖氧化酶氨基安替比林(COD—PAP)

  Experiment 28 Quantification of Blood Glucose by GlucoseOxidase4

  Aminoantipyrine(GOD—PAP)

  實驗29粗脂肪的提取和測定

  Experiment 29 Extraction and Quantitative DeterminationofCrude Fat

  實驗30  油脂的化學性質

  Experiment 30 Chemical Properties ofOil or Fat

第3部分  綜合性實驗

Part 3  Comprehensive Experiments

  實驗3l蛋白質的酸水解及其氨基酸的紙層析鑒定

  Experiment 3 1  Acid Hydrolysis of Proteins andIsolation and Identification

  ofAmino Acids by Paper Chromatography

  實驗32離子交換層析分離混合氨基酸

  Experiment 32  Separating Mixed Amino Acids byIon—exchange

  Chromatography

  實驗33血清球蛋白的鹽析分離及分子篩凝膠層析法脫鹽

  Experiment 33  Saltingout Separation of SerumGlobulins and Desalting by

  Molecular Sieving Gel Chromatography

  實驗34糖的測定和鑒定——蒽酮試劑和紙層析法

  Experiment 34 Quantification and Identification ofSaccharides by Anthrone

  Colorimetry and Paper Chromatography

  實驗35聚丙烯酰胺凝膠電泳分離乳酸脫氫酶同工酶

  Experiment 35  Separating LDH Isoenzymes by PAGE

  實驗36葉綠體的制備及相關分析

  Experiment 36 Preparation and Anabrses ofChloroplasts

  實驗37植物分子生物學系列實驗

  Experiment 37 Series Experiments ofPlant MolecularBiology

參考文獻

附錄

  附錄1  基礎生物化學實驗室常用儀器簡介

  附錄2  常用緩沖溶液的配制